FIGURE

Fig. 3

ID

ZDB-FIG-230115-60

Publication

Brandt et al., 2021 - Ablation of mpeg+ Macrophages Exacerbates mfrp-Related Hyperopia

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Fig. 3

Distribution of macrophage accumulation and ablation across mfrp⁺^/^–; and mfrp^–^/^– retinae. (A–H) Representative images of central retina sections from mfrp⁺^/^–; mpeg1:NTR-eYFP⁺ and mfrp^–^/^–; mpeg1:NTR-eYFP⁺ MTZ treated and untreated fish. (A–H) Grayscale DAPI images at low magnification (A, C, E, G) and high magnification (B, D, F, H). (A'–H') Grayscale eYFP images at low magnification (A', C', E', G') and high magnification (B', D', F', H'). (A''–H'') Grayscale Lcp1 images at low magnification (A'', C'', E'', G'') and high magnification (B'', D'', F'', H''). Colorized merged images with DAPI are shown in blue, eYFP in yellow, and Lcp1 in magenta; images are at low magnification (A''', C''', E''', G''') and high magnification (B''', D''', F''', H'''). (I–L) Quantification of the number of Lcp1⁺ cells per µm² in the ganglion cell layer (I) and inner (J) and outer (K) nuclear layer, and photoreceptor layer (L). (M–O) Quantification of the number of eYFP⁺ cells per µm² in the ganglion cell layer (M) and inner (N) and outer (O) nuclear layer. Error bars represent standard deviations. Two-way ANOVA was used for statistical analysis for (I) to (O). P values are shown from Tukey's multiple comparisons for post hoc analysis. ^*P < 0.05, ^*^*P < 0.01, ^**^*P < 0.001, ^***^*P < 0.0001. For mfrp⁺^/^– untreated, n = 4; for mfrp⁺^/^– MTZ treated, n = 4; for mfrp^–/– untreated, n = 4; for mfr^–/– MTZ treated, n = 5.

Expression Data

Antibodies:	Ab5-lcp1 AB9-GFP
Fish:	mfrp^mw78/+; w202Tg/w202Tg mfrp^mw78/mw78; w202Tg/w202Tg
Condition:	chemical treatment by environment: metronidazole
Anatomical Terms:	retina macrophage retinal ganglion cell layer retinal inner nuclear layer retinal outer nuclear layer
Stage:	Days 45-89

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mfrp^mw78/+; w202Tg/w202Tg mfrp^mw78/mw78; w202Tg/w202Tg
Condition:	chemical treatment by environment: metronidazole
Observed In:	retina macrophage retinal ganglion cell layer retinal ganglion cell layer macrophage retinal inner nuclear layer retinal inner nuclear layer macrophage retinal outer nuclear layer retinal outer nuclear layer macrophage
Stage:	Days 45-89

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Invest. Ophthalmol. Vis. Sci.