FIGURE

Fig. 2

ID

ZDB-FIG-220815-2

Publication

Ethiraj et al., 2022 - Colorimetric and fluorescent TRAP assays for visualising and quantifying fish osteoclast activity

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Fig. 2

A fluorescent TRAP method labels osteoclast activity in zebrafish bone fractures. A-L) Whole-mount widefield fluorescent images of adult fin ray fractures processed for ELF97 fluorescence. Fins were fixed at 3 dpc (A-C), 7 dpc (D-F), 14 dpc (G-I) and 21 dpc (J-L), and were either WT (A,D,G,J), homozygous csf1ra^j4e1 mutants (B, E, H, K) or treated with 100 μg/mL bisphosphonate (+BP; C, F, I, L). M: Mean fluorescence of crush sites at given timepoints and under the three conditions. ***= p<0.001, *=p<0.05, (ANOVA with Bonferroni multiple comparisons test, n=12). N: Mean ELF97 fluorescence at the crush sites of 1 dpc WT zebrafish following storage for 0, 1, 5, 7, 14, 21 days. There was no statistically significant variation over the course of 21 days of storage compared against initial staining (ANOVA, Tukey's multiple comparisons test, n=12). Scale bar: A) 100 μm.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Eur. J. Histochem.