Figure 1

Expression patterns of genes encoding amyloid and CNTN family members in zebrafish embryos.A and A′, cartoons representing the dorsal views of heads from zebrafish embryo at 24 and 48 h post fertilization (hpf). These views are similar to the whole-mount in situ hybridization analyses of amyloid and CNTN family members shown in B–G and B′–G′. B–B''', at 24 hpf, the colorimetric blue signal indicates the presence of aplp1 mRNA in the olfactory placode, telencephalon, and diencephalon. Although more widespread, the colorimetric signal remains in the olfactory placode at 48 hpf. C–C''', at 24 hpf, aplpl2 is expressed in the olfactory placode, eye lens, telencephalon, and diencephalon. The expression remains localized to these general areas at 48 hpf. D–D''', at 24 hpf, appb is expressed mostly in areas of the central nervous system and specifically in the telencephalon and diencephalon. It can also be detected in retinas at 48 hpf. However, no signal is observed in olfactory areas. E–E''', the expression of cntn4 is prevalent in the olfactory placode (24 hpf) and the olfactory pit (48 hpf) although a weak signal can be observed in the retina at 48 hpf. F–F''', cntn5 is found in the olfactory pit (48 hpf) and the telencephalon (24–48 hpf). G–G''', the expression of the gene eya1, which is known to be expressed in the olfactory placode and olfactory pit, is shown for comparison (18). For aplp1, B'' and B''' show detailed views of the olfactory regions denoted by boxes in B and B′, respectively. This panel arrangement is repeated for aplpl2, appb, cntn4, cntn5, and eya1. The scale bar represents 25 μm (24 hpf), 50 μm (48 hpf), and 10 μm in the detailed views of olfactory regions. CNTN, contactin.