FIGURE

Fig. S11

ID

ZDB-FIG-220203-63

Publication

Wang et al., 2020 - Specific fibroblast subpopulations and neuronal structures provide local sources of Vegfc-processing components during zebrafish lymphangiogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. S11

Fibroblasts at the horizontal myoseptum express Engrailed proteins. a-i) HM region of 48hpf embryos that were stained with anti-Engrailed (red) and anti-GFP (green) antibodies. a-c) All cells expressing the vegfc:Gal4FF; UAS: GFP reporter at the HM do co-express Engrailed proteins. d-f) fpdg-fra:mCitrine positive fibroblasts at the HM are also positive for Engrailed. g-i) Partial z-projections of the HM region reveal a co-expression of the adamts3 reporter and Engrailed proteins within fibroblasts. j, m) Two additional examples of genes whose transcripts are highly enriched within fibroblast cluster 2 are nuak1b and tgbl1 (see Supplementary Tables 1-3). The mRNA of both genes (k, l, n, o) can be detected specifically at the midline by ISH at 48hpf, thereby validating the notion that the cells in cluster 2 represent the fibroblast subpopulation located at the HM. Scale bars in a-i: 25 μm; k, l, n and o: 50 μm. hpf: hours post fertilization, HM: horizontal myoseptum

Expression Data

Genes:	itgbl1 nuak1b
Fish:	WT
Anatomical Term:	horizontal myoseptum fibroblast
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.