|
Single-cell RNA sequencing identifies fibroblast subpopulations expressing <italic>adamst3, adamts14, ccbe1</italic>, <italic>and vegfc</italic>.a Scheme of the partial dissociation approach and the work flow. Note that embryonic trunks were not completely dissociated in order to obtain a higher proportion of cells from more superficial tissues while reducing the amount of cells in the suspension originating from deeper tissues. b Pagoda2 clustering of the individual cell transcriptomes gives rise to 24 clusters (labeled as C1-C24). c–n t-SNE plots indicating the transcript levels of the respective gene in each sequenced cell. Expression of the general fibroblast markers pdgfra (c), col1a1a (d), lum (e), and dcn (f). Expression of ccbe1 (g) and vegfc (h) is largely restricted to cells within cluster 2 as is the case for the expression of the genes en1a (i) and en1b (j). adamts3- (k) and adamts14-expressing cells (l) are distributed over several clusters. m, n While cxcl12a-expressing cells are enriched in cluster 2, svep1-expressing cells can be found within several different clusters. t-SNE plots highlighting cells co-expressing vegfc and ccbe1 (o), adamts3 and vegfc (p), or adamts3 and ccbe1 (q) as well as cells co-expressing adamts14 and vegfc (r) or adamts14 and ccbe1 (s). Symbol shapes in the t-SNE plots indicate from which FACS run the cells had been obtained (circles and triangles originate from sorts using vegfc:Gal4FF; UAS:GFP; kdrl:mCherry embryos, cells indicated by squares stem from the FAC-sorting of pdgfra:mCitrine embryos), color scales reflect the relative transcript levels. hpf hours post fertilization.
|
