Differential expression of pharyngeal arch genes in the trrap mutants. Whole-mount in situ hybridization (WISH) analysis for dlx2a (a–d), dlx3 (e–h), and nkx2.3 (i–l) at 72 hpf. (a,c,e,g,i,k) Lateral views. (b,d,f,h,j,l) Ventral views. All pictures show the anterior aspect to the left. The expression of dlx2a (black arrowheads), dlx3 (red arrowheads) and nkx2.3 (double arrows) in the ventral pharyngeal arches was narrowed and restricted in the trrap mutants. The asterisks indicate the position of the mouth. After images were taken, genomic DNA was isolated from individual larvae, and genotyping was performed by genomic PCR. Scale bar, 200 μm.
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