Figure 2

The morpholino-mediated knock-down of fars2 delays vascular formation in zebrafish. (A) Representative images of the trunk regions of Tg (fli1: EGFP)^y1 embryos taken at 50 hpf. The intersegmental vessel (white arrow), dorsal longitudinal anastomotic vessel (blue arrow), parachordal vessel (red arrow), and ectopic sprouts (asterisks) are indicated. (B,C) The number of complete intersegmental vessels (ISVs) and the mean lengths of the ISVs in control and fars2 morphants. The horizontal bars show the mean ± SEM (n = 10 per group). ^***P < 0.001 via ANOVA. (D) Representative images of the caudal artery, caudal vein, and caudal vein plexus (CVP; arrows) in Tg (fli1: EGFP)^y1 embryos taken at 50 hpf. In the control embryo, the CVP formed a typical honeycomb structure in the tail (white arrows). The knock-down of fars2 resulted in specific defects in CVP formation (yellow arrows). (E) Quantification of the loop number at the CVP. The horizontal bars show the mean ± SEM (n = 10 per group). ^***P < 0.001 via ANOVA.