FIGURE 6

Amino acids significantly altered upon HRAS^G12V induction in PNCs. (A) Box and whisker plots generated using MetaboAnalyst 5.0 online platform for arginine and proline metabolism, identified using pathway enrichment analysis. (B) Variable importance in projection (VIP) plot generated using MetaboAnalyst 5.0 online platform for the 15 amino acids identified in our dataset. The relative abundance of each amino acid in the preneoplastic cell (PNC) and control (WT) groups are presented as colored boxes ranging from blue (low) to red (high). The average for N = 10 is presented. (C) Box and whisker plots for the four amino acids with a VIP cut-off score of 1, except hydroxyproline and proline presented in (A). The altered metabolites with statistical significance (p < 0.05) are presented in blue and the non-significant metabolites in gray. The boxplots represent median ± interquartile range of log transformed and auto-scaled intensities for preneoplastic cell (PNC) and control (WT) groups (N = 10).