FIGURE 4

Metabolic pathways significantly altered upon HRAS^G12V induction in PNCs. (A) Partial Least Squares—Discriminant Analysis (PLS-DA) score plot shows separation of the preneoplastic cell (PNC) and control (WT) groups. The first and second components explain 9.2 and 12.8% of the variation, respectively. Different colors and shapes correspond to PNC, red circle and WT, blue square. (B) Scatter plot of Danio rerio KEGG metabolic pathways significantly altered upon HRAS^G12V induction. The p-values from the pathway enrichment analysis are shown in darker color indicating more significant changes within a pathway, while the pathway impact values from the pathway topology analysis are depicted by the size of the node. Significantly (p < 0.05) impacted pathways are numbered and tabulated: 1, Taurine and hypotaurine metabolism; 2, Glutathione metabolism; 3, Pyrimidine metabolism; 4, Valine, leucine and isoleucine degradation; 5, Arginine and proline metabolism; 6, Purine metabolism. The match values indicate the number of metabolites identified within the KEGG pathway.