PUBLICATION
Metabolic Alterations in Preneoplastic Development Revealed by Untargeted Metabolomic Analysis
- Authors
- Myllymäki, H., Astorga Johansson, J., Grados Porro, E., Elliot, A., Moses, T., Feng, Y.
- ID
- ZDB-PUB-210821-8
- Date
- 2021
- Source
- Frontiers in cell and developmental biology 9: 684036 (Journal)
- Registered Authors
- Feng, Yi
- Keywords
- HRAS, cancer metabolism, metabolome, preneoplastic, untargeted metabolomics, zebrafish
- MeSH Terms
- none
- PubMed
- 34414180 Full text @ Front Cell Dev Biol
Citation
Myllymäki, H., Astorga Johansson, J., Grados Porro, E., Elliot, A., Moses, T., Feng, Y. (2021) Metabolic Alterations in Preneoplastic Development Revealed by Untargeted Metabolomic Analysis. Frontiers in cell and developmental biology. 9:684036.
Abstract
Metabolic rewiring is a critical hallmark of tumorigenesis and is essential for the development of cancer. Although many key features of metabolic alteration that are crucial for tumor cell survival, proliferation and progression have been identified, these are obtained from studies with established tumors and cancer cell lines. However, information on the essential metabolic changes that occur during pre-neoplastic cell (PNC) development that enables its progression to full blown tumor is still lacking. Here, we present an untargeted metabolomics analysis of human oncogene HRASG12V induced PNC development, using a transgenic inducible zebrafish larval skin development model. By comparison with normal sibling controls, we identified six metabolic pathways that are significantly altered during PNC development in the skin. Amongst these altered pathways are pyrimidine, purine and amino acid metabolism that are common to the cancer metabolic changes that support rapid cell proliferation and growth. Our data also suggest alterations in post transcriptional modification of RNAs that might play a role in PNC development. Our study provides a proof of principle work flow for identifying metabolic alterations during PNC development driven by an oncogenic mutation. In the future, this approach could be combined with transcriptomic or proteomic approaches to establish the detailed interaction between signaling networks and cellular metabolic pathways that occur at the onset of tumor progression.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping