Polymerization and potential ubiquitin E3 ligase function are indispensable for Irf2bp2a in regulating the differentiation of neutrophilss.(A) Structure, missing function and rescue effect of wild type and variant forms of Irf2bp2a. (B-J) Irf2bp2a mRNA rescue assays in irf2bp2a-/- embryos. Mpx probe was used in WISH to examine rescue effect with wild irf2bp2a (D), ZM (E), Δzinc finger (F), tet-ZM (G), RM (H), Δring finger (I) and K486R mutant (J) mRNA injections. (K) The statistical significance was calculated by using one-way ANOVA. The statistical significance was calculated using 1-way ANOVA followed by Dunnett T3 correction. The asterisk indicates a statistical difference (N = 5, 15–25 embryos were used for each experiment. Each dot represents the mean value of one experiment. Error bars represent mean ± SEM. ns: no statistical significance; ***P < 0.001).
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