FIGURE

Figure 3

ID

ZDB-FIG-210801-8

Publication

Li et al., 2021 - Disruption of MAP7D1 Gene Function Increases the Risk of Doxorubicin-Induced Cardiomyopathy and Heart Failure

Other Figures

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Figure 3

The GBT239/map7d1b homozygous mutant exhibited increased apoptotic cardiomyocyte death concurrent with impaired autophagy and elevated protein aggregation upon doxorubicin stress. (a, b) Representative images of the TUNEL assay (a) and quantification of the percentage of TUNEL-positive nuclei in GBT239/map7d1b homozygous mutant (map7d1b-/-) compared to WT controls at 4 weeks postdoxorubicin injection. n=4, Student’s t-test. Scale bar: 20 μm. (c, d) Representative Western blot images (c) and quantification analysis (d) of the expression levels of autophagy molecular markers LC3II and p62 (SQSTM1), ER stress marker glucose-regulated protein 78 (Grp78), and ubiquitin aggregated protein examined in the heart tissues isolated from the map7d1b-/- mutant compared to WT control at 4 weeks postdoxorubicin injection. n=3, Student’s t-test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	map7d1b^{mn0239Gt/mn0239Gt}
Condition:	chemical treatment by injection: doxorubicin
Observed In:	autophagic cell death cardiac muscle cell
Stage:	Adult

Phenotype Detail

Acknowledgments

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