Fig. 2

Multiple transgenic lines derived from independent founder (F0) parental fish were established that transmit the peak5:betaglobin:EGFP reporter through the germline. White arrowheads indicate NCCs. Black arrowheads point to KA neurons. Asterisks indicate heart EGFP localization in the heart. a Stable line A exhibits strong EGFP localization in NCCs at 1 dpf. At 2 dpf, some NCCs are labeled, and KA neurons are also faintly visible. At 5 dpf, the most prominently labeled cells are KA neurons and cells near or within the swim bladder. b At 1 dpf, Line B exhibits EGFP localization in posterior premigratory NCCs and strong localization in KA neurons. By 3 and 5 dpf, EGFP localization is primarily within KA neurons and the heart. c Line C mainly exhibits EGFP localization in KA neurons at 1, 2, and 3 dpf. d Lightsheet microscopy of the dorsal aspect of peak5:betaglobin:EGFP Line A embryos shows peak5 is active in cells with NCC morphology and premigratory NCC localization at 1 dpf and d′–d″ co-labels with Tg(sox10(7.2):mRFP) positive cells. Arrowheads point to representative co-labeled cells. The lateral aspect also shows e–e″ EGFP positive cells that co-localize with mRFP positive cells. Arrowheads point to representative co-labeled cells. f Whole animal image of peak5 active in stable transgenic line A. Asterisks (*) indicate regions of auto-fluorescence; a long pass filter set allows discrimination between the yellow hue of autofluorescent tissue and EGFP-expressing cells. g EGFP positive cells peripheral nervous system cells within the barbel (arrowhead).