FIGURE

Fig. 1

ID

ZDB-FIG-210612-7

Publication

Cunningham et al., 2021 - Functional in vivo characterization of sox10 enhancers in neural crest and melanoma development

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Fig. 1

ATAC-Seq of zebrafish melanoma tumors identifies <italic>peak5</italic> as an active neural crest enhancer.

a Schematic for obtaining zebrafish melanoma tumor cells for ATAC-Seq. b Regions of open chromatin surrounding the sox10 locus. Peak numbers are annotated below. MP minimal promoter. c Schematic for screening putative enhancers for activity in NCCs and melanoma. dpeak5-driven EGFP expression is mosaically present in NCCs (arrowheads) in F0 injected embryos, as indicated by co-localization with crestin:mch at 1 dpf. e The sox10 minimal promoter in F0 embryos is active in both NCCs (arrowheads) and the CNS (asterisk). f F0 embryos injected with a negative control (~76 kb upstream of the sox10 TSS) exhibit only limited expression of EGFP not localized to NCCs. Insets show enlarged region above yolk extension.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tp53^zdf1/zdf1; cz3337Tg/cz3337Tg; czt13Tg/czt13Tg
Observed In:	integument melanoma positive regulation of core promoter binding
Stage:	Adult

Phenotype Detail

Acknowledgments

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