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Fig. 2

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ZDB-IMAGE-210612-21
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Figures for Cunningham et al., 2021
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Fig. 2 <italic>peak5</italic> stable transgenic lines exhibit similar expression patterns in subsets of NCCs and neural crest derivatives.

Multiple transgenic lines derived from independent founder (F0) parental fish were established that transmit the peak5:betaglobin:EGFP reporter through the germline. White arrowheads indicate NCCs. Black arrowheads point to KA neurons. Asterisks indicate heart EGFP localization in the heart. a Stable line A exhibits strong EGFP localization in NCCs at 1 dpf. At 2 dpf, some NCCs are labeled, and KA neurons are also faintly visible. At 5 dpf, the most prominently labeled cells are KA neurons and cells near or within the swim bladder. b At 1 dpf, Line B exhibits EGFP localization in posterior premigratory NCCs and strong localization in KA neurons. By 3 and 5 dpf, EGFP localization is primarily within KA neurons and the heart. c Line C mainly exhibits EGFP localization in KA neurons at 1, 2, and 3 dpf. d Lightsheet microscopy of the dorsal aspect of peak5:betaglobin:EGFP Line A embryos shows peak5 is active in cells with NCC morphology and premigratory NCC localization at 1 dpf and d′–d″ co-labels with Tg(sox10(7.2):mRFP) positive cells. Arrowheads point to representative co-labeled cells. The lateral aspect also shows ee″ EGFP positive cells that co-localize with mRFP positive cells. Arrowheads point to representative co-labeled cells. f Whole animal image of peak5 active in stable transgenic line A. Asterisks (*) indicate regions of auto-fluorescence; a long pass filter set allows discrimination between the yellow hue of autofluorescent tissue and EGFP-expressing cells. g EGFP positive cells peripheral nervous system cells within the barbel (arrowhead).

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