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Effect of dachb knockdown on the expression of dach genes. Embryos were either uninjected or they were injected with 4 ng p53-MO or with 4 ng splice-blocking dachb-morpholino (dachb-MO-S) + 4 ng p53-MO and then the reverse transcription polymerase chain reaction was carried out to determine any changes in the expression of dacha, dachb, dachc and dachd. As expected, the expression of dachb was downregulated significantly in the p53-MO + dachb-MO group when compared with the two control groups. There was also a significant reduction of dacha expression compared with the control groups. The data are presented as the mean ± standard error of four experiments per treatment. The asterisks indicate p53-MO + dachb-MO data that are significantly different from the p53-MO alone injected controls for each of the four genes at P < 0.01** or P < 0.001*** using the Student’s t-test. NS, not significant.
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