Fig. 6

Injection of dachb messenger ribonucleic acid (mRNA) rescues the effect of splice-blocking dachb-morpholino (dachb-MO-S) on the development of the pancreatic islet in embryos at 30 h post-fertilization (hpf). Embryos were (a) uninjected (control) or they were injected at the one-cell stage with (b) p53-MO (control), (c) 2 ng dachb-MO-S + 2 ng p53-MO or (d) 2 ng dachb-MO-S + 2 ng p53-MO + 50 pg dachb mRNA. (ai–di) Bright-field images were acquired at 30 hpf, after which the embryos were whole-mount double-immunolabeled with antibodies to insulin (green) and islet 1 and 2 (red) to show the localization of the β-cells and islet cells in the endocrine pancreas, respectively. (aii–dii) These are all single optical sections taken through the middle of pancreatic islet. (e,f) The number of (e) β-cells and (f) islet cells, and (g,h) the volume of (g) β-cells and (h) islet cells. (i) The circularity of the pancreatic islet. The data are presented as the mean ± standard error of n = 10 to 28 embryos, such that the number is shown in the respective bars in panel (e). The asterisks indicate significant differences at P < 0.05*, P < 0.01** or P < 0.001*** using the Mann–Whitney U-test. Ant., anterior; D, dorsal; NS, not significant; Pos. posterior; V, ventral.