Figure 7 - figure supplement 2

Inhibiting Rho kinase blocks caudal venous plexus (CVP) cavernoma formation in <italic toggle='yes'>ccm2</italic> CRISPR zebrafish.

At 24 hpf, ccm2 CRISPR embryos were treated with 45 μM Y-27632(Tocris) in egg water as described by Uehata et al. (Nature:389 (1997) pp. 990–994) and controls were treated with 1% DMSO. At 2 days post fertilization (dpf), in contrast to DMSO-treated embryos, no embryos treated with Y-27632 exhibited CVP dilation (**p=0.0098) or other obvious developmental anomalies. Total number of embryos in each group is indicated above the bars. Two-tailed Fisher’s exact test was used for comparisons.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Phenotype Detail
Acknowledgments
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