FIGURE

Figure 5

ID

ZDB-FIG-210409-5

Publication

Kayman Kürekçi et al., 2021 - Knockout of zebrafish desmin genes does not cause skeletal muscle degeneration but alters calcium flux

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Figure 5

Desmin protein expression and histological examination of adult skeletal muscle. (a) Photographs of 1 year-old anesthetized fish. (b) Immunoblotting of skeletal muscle protein extracts of WT, desma^kg97/kg97, desma^kg97/+, desmb^kg156/kg156, desmb^kg156/+ and desma^kg97/kg97;desmb^kg156/kg156 double mutant fish probed with anti-desmin antibody. Lamin B1 was labeled on the same blot as loading control. Uncropped full-length pictures of the blot is available in Supplementary Figure S5 online. (c) Transversal cryosections of mid-trunk somites from two different areas of WT and homozygous mutant adults stained with hematoxylin eosin showing no pathological changes. Scale bar: 25 µm.

Expression Data

Antibodies:	Ab1-desm Ab5-lmnb2
Fish:	AB desma^kg97/kg97 (AB) desmb^kg156/kg156 (AB) desma^kg97/+ (AB) desmb^kg156/+ (AB) desmb^kg156/kg156; desma^kg97/kg97 (AB)
Anatomical Term:	skeletal muscle
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	desma^kg97/kg97 (AB) desmb^kg156/kg156 (AB) desmb^kg156/kg156; desma^kg97/kg97 (AB)
Observed In:	skeletal muscle trunk muscle cell whole organism
Stage:	Adult

Phenotype Detail

Acknowledgments

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