Fig. 5

a, b Expression of alk2, alk3, alk6a, bmpr2a, bmpr2b, actr2a, and actr2b mRNA in ZFL cells stimulated with poly(I:C) (2 μg/ml, a) or GCRV (5 × 10⁴TCID₅₀ per ml, b) for 48 h. c–e Expression of ifnφ1 (c) and ifnφ3 (d) mRNA in ZFL cells or EPC ifn (e) in EPC cells which were transfected with 2 μg of alk2, alk3, alk6a, bmpr2a, bmpr2b, actr2a, actr2b or empty vector for 48 h. f, h Expression of irf3, irf7, tbk1, ifn (or ifnφ1 and ifnφ3), and mx mRNA after transfected with 2 μg of pcDNA3.1-alk6a or empty vector in ZFL (f) or EPC (h) cells for 48 h. g, i Expression of irf3, irf7, tbk1, ifn (or ifnφ1 and ifnφ3), and mx mRNA after transfected with 2 μg of of pcDNA3.1-alk6a or empty vector in ZFL (g) or EPC (i) cells for 24 h, followed by infection with GCRV for another 36 h. j Schematic drawing of the alk6a-ΔGS mutation that the GS domain of Alk6a was deleted. k, m Expression of irf3, irf7, tbk1, ifn (or ifnφ1 and ifnφ3), and mx mRNA after transfected with 2 μg pcDNA3.1-alk6a-ΔGS or empty vector in ZFL (k) or EPC (m) cells for 48 h. l, n Expression of irf3, irf7, tbk1, ifn (or ifnφ1 and ifnφ3), and mx mRNA after transfected with 2 μg pcDNA3.1-alk6a-ΔGS or empty vector in ZFL (l) or EPC (n) cells for 24 h, followed by infection with GCRV for another 36 h. The expression of zebrafish actb1 or EPC actin was used as an internal control for the qRT-PCR. Data were from three independent experiments and were analyzed by Student’s t-test (two-tailed) for comparison of two groups or one-way ANOVA followed by Games–Howell posthoc tests for comparison of multiple groups. All data were presented as mean ± SD (*p < 0.05, **p < 0.01, and *** p < 0.001, ns means no significant difference).