FIGURE

Fig. 5

ID

ZDB-FIG-210301-152

Publication

Zimmer et al., 2020 - Use of a carbonic anhydrase ca17a knockout to investigate mechanisms of ion uptake in zebrafish (Danio rerio)

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Fig. 5

The effect of Ca17a knockdown on chloride (Cl⁻) uptake in 4-dpf zebrafish (Danio rerio) larvae reared in low-Cl⁻ water and the effect of rearing in low-Cl⁻ water on ca17a protein abundance in 4-dpf larvae. There was an effect of ca17a knockdown and rearing water Cl⁻ concentration on Cl⁻ uptake (A) (two-way ANOVA; Cl⁻ concentration: F = 51.9, P < 0.01, morpholino: F = 110.1, P < 0.01, Cl⁻ concentration × morpholino: F = 13.6, P < 0.01, n = 7). There was an effect of rearing water Cl⁻ concentration on Ca17a protein abundance (B) (Welch’s two-sample t test; t = −2.8, P = 0.02, n = 6). Different letters signify significant (P < 0.05) differences between sham and morpholino larvae within a rearing water Cl⁻ concentration and bars with an asterisk indicate significant effects of rearing water Cl⁻ concentration. Data are presented as means ± SE.

Expression Data

Gene:	ca2
Fish:	WT
Condition:	water salinity
Anatomical Term:	whole organism
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO4-ca2
Condition:	water salinity
Knockdown Reagent:	MO4-ca2
Observed In:	whole organism whole organism chloride transport
Stage:	Day 4

Phenotype Detail

Acknowledgments

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