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Fig. 5 The effect of Ca17a knockdown on chloride (Cl−) uptake in 4-dpf zebrafish (Danio rerio) larvae reared in low-Cl− water and the effect of rearing in low-Cl− water on ca17a protein abundance in 4-dpf larvae. There was an effect of ca17a knockdown and rearing water Cl− concentration on Cl− uptake (A) (two-way ANOVA; Cl− concentration: F = 51.9, P < 0.01, morpholino: F = 110.1, P < 0.01, Cl− concentration × morpholino: F = 13.6, P < 0.01, n = 7). There was an effect of rearing water Cl− concentration on Ca17a protein abundance (B) (Welch’s two-sample t test; t = −2.8, P = 0.02, n = 6). Different letters signify significant (P < 0.05) differences between sham and morpholino larvae within a rearing water Cl− concentration and bars with an asterisk indicate significant effects of rearing water Cl− concentration. Data are presented as means ± SE.