FIGURE

Fig. 8

ID

ZDB-FIG-210301-155

Publication

Zimmer et al., 2020 - Use of a carbonic anhydrase ca17a knockout to investigate mechanisms of ion uptake in zebrafish (Danio rerio)

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Fig. 8

Gas transfer, respiratory exchange ratio (RER; /Ṁo₂) and ammonia excretion in ca17a+/+, ca17a+/−, and ca17a−/− zebrafish (Danio rerio) larvae at 4 dpf and 9 dpf. There was no effect of genotype on O₂ consumption (A) (Ṁo₂; ANOVA; 4 dpf: F = 1.7, P = 0.19; 9 dpf: F = 0.7, P = 0.49), CO2 excretion (B) (Ṁco2; ANOVA; 4 dpf: F<0.1, P = 0.99; 9 dpf: F = 0.3, P = 0.72), or RER (C) (ANOVA; 4 dpf: F = 0.9, P = 0.42; 9 dpf: F = 2.1, P = 0.15) in 4 and 9 dpf larvae (4 dpf: n = 12 for ca17a+/+ and ca17a−/−, n = 10 for ca17a+/−; 9 dpf: n = 8 for ca17a+/+ and ca17a−/−, n = 7 for ca17a+/−). There was an effect of genotype on ammonia excretion in 4-dpf (D) (ANOVA; F = 6.1, P < 0.01, n = 9 for ca17a+/+ and ca17a+/−, n = 8 for ca17a−/−) but not 9-dpf larvae (ANOVA; F = 3.1, P = 0.07, n = 9 for ca17a+/+ and ca17a+/−, n = 6 for ca17a−/−). Bars with different letters are significantly different (P < 0.05) from one another across genotypes. Data are presented as means ± SE. dpf, days postfertilization.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ca2^ot206/ot206 ca2^ot206/+
Observed In:	ammonium excretion respiratory gaseous exchange by respiratory system
Stage Range:	Day 4 to Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.