Figure 3. The rbp1a Gene Plays a Role Downstream of the lrp5 Gene (A) qPCR analysis showed that the expression level of the rbp1a gene was remarkably decreased in the lrp5−/− (~11%) and digenic eys+/-; lrp5+/− (~7%) eyes while moderately decreased in the eys−/− eyes (~51%) compared with wild-type eyes at 3.5 mpf. (B and C) There was no change in the expression level of either the prom1a or prom1b gene in the eys−/− eyes compared with wild-type eyes at 3.5 mpf. (D) The expression level of the lrp5 gene was not remarkably changed in the rbp1a−/− eyes (~142%) compared with wild-type eyes at 3.5 mpf. The rbp1a gene played a role downstream of the lrp5 gene (A and D). (E) The expression level of the eys gene was slightly increased in the prom1a−/−; prom1b−/− eyes (~229% for 5′ region, ~166% for 3′ region, and ~155% for splice variant 3 [Takita et al., 2019]) compared with wild-type eyes at 3.5 mpf. Negative genetic interaction between the eys gene and the prom1a or prom1b gene was not observed (B, C, and E). All data are represented as mean ± SD. ∗p < 0.005, ∗∗p < 0.0005, ∗∗∗p < 0.0001, ∗∗∗∗p < 0.000001. Ns, not significant.
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