Sulf2b is expressed within Shh-producing cells whereas Sulf2a is restricted to neural progenitors. Here and in all subsequent panels, side views of embryos (a,c,e,g,k,l) are at the level of the trunk spinal cord, anterior to the left and dorsal to the top, and spinal cord transverse sections (b,d,f,h,i,j) are shown dorsal to the top. Developmental stages are indicated in each panel. (a–j) Detection of sulf2b (a–d) and sulf2a (e–j) mRNAs by whole-mount in situ hybridisation. Images are representative examples of at least five embryos. Grey outlines (b,d,f,h,i,j) label the spinal cord edge. Brackets in a,c,e,g indicate the position of the spinal cord. Dotted lines in b,d,f,h) delineate Shh-producing cells, i.e. the medial floor plate at 24 hpf and the medial floor plate + lateral floor plat at 36 hpf. Note that sulf2a mRNA is detected in neural progenitors but not in medial and lateral floor plate cells. (k-l) Double detection of sulf2a (red) and shh (green) mRNAs by fluorescent in situ hybridisation, showing that sulf2a expression does not overlap the shh-expressing domain (white brackets).
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