FIGURE

Figure 7

ID

ZDB-FIG-201229-70

Publication

Keefe et al., 2020 - Vanishing white matter disease expression of truncated EIF2B5 activates induced stress response

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Figure 7

Human EIF2B2 gene rescues zebrafish eif2b2^{sa17223/sa17223} mutants. (A) 11.1 kb zebrafish eif2b2 gene structure. (B) 9.5 kb human EIF2B2 gene structure. (C) Conservation of amino acid sequence between zebrafish eif2b2 and human EIF2B2. (D) Schematic of rescue construct containing Tol2, β-actin, human EIF2B2, and eGFP. (E) Genotype results (at adult age), of an eif2b2^sa17223/+ heterozygous incross, and an eif2b2^sa17223/+ heterozygous zebrafish crossed with two different transgenic alleles (#3 and #4): eif2b2^sa17223/+;Tg3(β-actin:EIF2B2:2A:eGFP) or eif2b2^sa17223/+;Tg4(β-actin:EIF2B2:2A:eGFP) heterozygous zebrafish. (F) Bright-field and immunofluorescent images of eif2b2^{sa17223/sa17223};β-actin:EIF2B2:2A:eGFP mutant fish: showing a swim bladder and regular-sized head; or showing GFP expression in eif2b2^{sa17223/sa17223};β-actin:EIF2B2:2A:eGFP mutant fish. (G–J) TUNEL and DAPI antibody staining of wild-type control; eif2b2^{sa17223/sa17223} mutant; eif2b2^+/+;β-actin:EIF2B2:2A:eGFP wild-type control; and eif2b2^{sa17223/sa17223};β-actin:EIF2B2:2A:eGFP mutant. (K) Quantification of TUNEL+ cells.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	eif2b2^{sa17223/sa17223} eif2b2^{sa17223/sa17223}; vu19Tg
Observed In:	brain apoptotic process head swim bladder
Stage:	Day 6

Phenotype Detail

Acknowledgments

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