FIGURE

Figure 2

ID

ZDB-FIG-201003-186

Publication

Houseright et al., 2020 - Cell type specific gene expression profiling reveals a role for complement component C3 in neutrophil responses to tissue damage

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Figure 2

TRAP-RNAseq identifies upregulation of the complement pathway and c3a.1 in response to wounding. (A) Expression measured by RNA-seq (fpkm) of complement pathway gene c3a.1 across all three cell types. Each dot represents one replicate. (B) RT-qPCR validation of c3a.1 gene expression in neutrophils and macrophages. Each dot represents one replicate; measurements for each replicate were performed in triplicate. Data is expressed as the fold change in mean Cq, normalized to ef1a expression and expression of c3a.1 in unwounded controls. N = 200 larvae/condition/replicate. C, D. Expression measured by RNA-seq (fpkm) of complement pathway genes c5 (C) and c9 (D), across all 3 three cell types. Each dot represents one replicate.

Expression Data

Genes:	c3a.1 c5 c9
Fish:	uwm15Tg uwm16Tg uwm17Tg
Condition:	transection: caudal fin
Anatomical Terms:	epithelial cell macrophage neutrophil
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	uwm15Tg uwm16Tg
Condition:	transection: caudal fin
Observed In:	macrophage neutrophil
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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