FIGURE

Fig. 3

ID

ZDB-FIG-200910-36

Publication

Chetty et al., 2020 - Ets1 functions partially redundantly with Etv2 to promote embryonic vasculogenesis and angiogenesis in zebrafish

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Fig. 3

Loss of Ets1 function impairs vasculogenesis in Etv2-deficient embryos. (A–D) Confocal micrographs of 28 hpf Tg(kdrl:GFP; kdrl:nls-mCherry) wild-type, ets1−/−, etv2 MO (0.25 ng) and ets1−/−; etv2 MO (0.25 ng) zebrafish embryos (trunk region). (E) Quantitative analysis of endothelial cell number in the axial vessels of embryos in A-D by cell counts in Imaris. (F) FACS analysis of GFP + endothelial cells in 28 hpf embryos. Note the decrease in endothelial cell number between etv2 MO and ets1−/−; etv2 MO embryos (E,F). Lateral views; anterior is to the left. ∗P < 0.05, ∗∗∗P < 0.001; n.s – not significant; error bars represent ± SEM.

Expression Data

Genes:	EGFP mCherry
Fish:	is4Tg; s843Tg ets1^ci14/ci14; is4Tg; s843Tg is4Tg; s843Tg + MO2-etsrp ets1^ci14/ci14; is4Tg; s843Tg + MO2-etsrp
Knockdown Reagent:	MO2-etsrp
Anatomical Terms:	blood vasculature blood vessel blood vessel endothelial cell nucleus intersegmental vessel
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ets1^ci14/ci14; is4Tg; s843Tg is4Tg; s843Tg + MO2-etsrp ets1^ci14/ci14; is4Tg; s843Tg + MO2-etsrp
Knockdown Reagent:	MO2-etsrp
Observed In:	axial blood vessel blood vessel endothelial cell blood vasculature blood vessel intersegmental vessel
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 465(1), Chetty, S.C., Sumanas, S., Ets1 functions partially redundantly with Etv2 to promote embryonic vasculogenesis and angiogenesis in zebrafish, 11-22, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.