FIGURE

Fig. 4

ID

ZDB-FIG-200910-37

Publication

Chetty et al., 2020 - Ets1 functions partially redundantly with Etv2 to promote embryonic vasculogenesis and angiogenesis in zebrafish

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Fig. 4

Ets1 promotes endothelial cell survival in axial vessels. (A-D″) Tg(kdrl:GFP), ets1−/−, etv2−/− and ets1−/−; etv2−/−embryos at 24 hpf immuno-stained for apoptosis marker Cleaved Caspase3 and vascular GFP. Note the normal vasculature in ets1−/−embryos (B) despite extensive Caspase 3 staining in the axial vessels (B′). Both etv2−/− (C-C″) and ets1−/−; etv2−/−embryos (D-D″) exhibit severe vascular defects and extensive apoptosis in the trunk region. (E) Quantification of apoptotic cells within trunk vasculature in 24 hpf embryos. Note the increase in apoptotic cells in the ets1−/−; etv2−/− embryos compared to etv2−/− embryos. ∗P < 0.05, ∗∗P < 0.01; horizontal bars represent mean, error bars represent ± SEM.

Expression Data

Antibodies:	Ab1-casp3 Ab16-GFP
Fish:	s843Tg ets1^ci14/ci14; s843Tg etsrp^y11/y11; s843Tg ets1^ci14/ci14; etsrp^y11/y11; s843Tg
Anatomical Terms:	blood vasculature blood vessel blood vessel endothelial cell intersegmental vessel
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ets1^ci14/ci14; s843Tg etsrp^y11/y11; s843Tg ets1^ci14/ci14; etsrp^y11/y11; s843Tg
Observed In:	blood vasculature blood vessel blood vessel endothelial cell blood vessel endothelial cell apoptotic process intersegmental vessel
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 465(1), Chetty, S.C., Sumanas, S., Ets1 functions partially redundantly with Etv2 to promote embryonic vasculogenesis and angiogenesis in zebrafish, 11-22, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.