FIGURE

Fig. 1

ID

ZDB-FIG-200730-8

Publication

Hall et al., 2020 - In vivo cell biological screening identifies an endocytic capture mechanism for T-tubule formation

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Fig. 1

An in vivo system for T-tubule development.

a–e The CaaX domain from Hras marks both sarcolemma and T-tubule. Markers were screened at 48 hpf for T-tubule specificity on a stable transgenic background expressing Bin1b-Citrine from the endogenous locus. a CaaX domain from Hras (tH). b CaaX domain from Kras (tK). c Myristoylation domain from ARF5. d BODIPY-TR vital dye. e GPI anchor from zebrafish folate receptor gamma. f Time course of tubule formation using stable EGFP-CaaX background. All images are representative of 12 individual cells within different individual animals. See also Supplementary Movies 1 and 2. Scale bars; (a–e) 10 µm, (f) 50 μm.

Expression Data

Genes:	EGFP mKate2
Fish:	pc10Tg uq22rpTg
Anatomical Terms:	skeletal muscle sarcolemma skeletal muscle T-tubule
Stage Range:	14-19 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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