FIGURE

Fig. 9

ID

ZDB-FIG-200614-11

Publication

Castro et al., 2020 - Hcfc1a regulates neural precursor proliferation and asxl1 expression in the developing brain

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Fig. 9

Quantification of the number of NPCs after inhibition of Asxl1 activity. a, b Quantification of the number of Sox2⁺ (a) or EdU (b) positive cells in vehicle treated and larvae treated with LY294002 at 2 days post fertilization (DFP). N = 4 Vehicle Sibling, 6 Vehicle hcfc1a^co60/+ larvae, and 4 LY294002 hcfc1a^co60/+ larvae. ^§p = 0.000168, ^§§p = 2.08852E−09, ^§§§p = 0.000153. ^◊p = 7.22142E−07, ^◊◊p = 0.000997, ^◊◊◊p = 0.00014. All error bars represent standard error of the mean. chcfc1a^co60/+ larvae and their wildtype siblings (Sibling) were treated at 24 h intervals with 12 μM LY294002 until 5 DPF and then total RNA was isolated from brain homogenates. Quantitative real time PCR was performed to test the expression of sox2, asxl1, and cyclin E (ccne1). N = 10/group/biological replicate. Error bars represent standard error of the mean. *p < 0.05

Expression Data

Genes:	asxl1 ccne1 sox2
Fish:	WT hcfc1a^co60/+
Condition:	chemical treatment by environment: EC 2.7.1.137 (phosphatidylinositol 3-kinase) inhibitor
Anatomical Term:	brain
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hcfc1a^co60/+
Condition:	chemical treatment by environment: EC 2.7.1.137 (phosphatidylinositol 3-kinase) inhibitor
Observed In:	brain brain neural precursor cell proliferation brain neuronal stem cell
Stage Range:	Long-pec to Day 5

Phenotype Detail

Acknowledgments

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