FIGURE

Fig. 1

ID

ZDB-FIG-200614-3

Publication

Castro et al., 2020 - Hcfc1a regulates neural precursor proliferation and asxl1 expression in the developing brain

Other Figures

(all 10)

All Figure Page

Back to All Figure Page

Fig. 1

Haploinsufficiency of the hcfc1a^co60/+ allele (Co60/+). a Schematic of the exon structure of the hcfc1a gene (not drawn to scale). The gRNA produced targets the sequence shown within exon 3 (Sibling). The Co60 allele results in an insertion of 13 nucleotides (Red). b Primers were designed to specifically amplify the Co60 allele. The specific primer is underlined and bolded in the sequence presented in (a). Positive amplification is indicative of heterozygous carriers (Co60/+) with no amplification of the wildtype allele (WT). c Quantitative real time PCR analyzing the relative expression of hcfc1a in wildtype siblings (Sibling) and the hcfc1a^co60/+ allele (Co60/+) at 2 days post fertilization. N = 16 larvae per group with three biological replicates. *p < 0.05

Expression Data

Gene:	hcfc1a
Fish:	WT hcfc1a^co60/+
Anatomical Term:	whole organism
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hcfc1a^co60/+
Observed In:	whole organism
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Neurosci.