FIGURE

Fig. 4

ID

ZDB-FIG-200413-43

Publication

Taler et al., 2019 - Lysyl hydroxylase 3 is required for normal lens capsule formation and maintenance of lens epithelium integrity and fate

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Fig. 4

pnina is allelic to plod3. (A,B) Transverse histological sections through eyes of 3 dpf normal sibling (A) and plod3^tv205/tv205 (B) embryos (n = 10 embryos from each genotype). (C,D) Live 3 dpf embryos from a cross between pnina^vu222/+ and plod3^tv205/+ fish. From over 200 embryos, approximately 75% of embryos appeared normal (top embryo) and 25% showed a plod3 mutant-like phenotype (bottom embryo). C is lateral view, D, dorsal view (compare to Fig. 1A,B’,C’). (E) Relative plod3 expression as measured by RT-qPCR in plod3^vu222/vu222 mutant and normal embryos. Blue lines show mean expression level (relative quantity, RQ) and bars represent highest and lowest expression levels based on the standard error of the delta Ct’s. (F,G) Transverse sections through eyes of 30 hpf (F) and 48 hpf (G) wild-type embryos labeled for plod3 expression by in situ RNA hybridization (n = 10 eyes for each time point). Arrows in E point at the ALE and in F at CMZs. Scale bars are 50 μm (A,B), 1 mm (C), 100 μm (D), 20 μm (F,G).

Expression Data

Gene:	plod3
Fish:	WT plod3^vu222/vu222
Anatomical Terms:	ciliary marginal zone lens epithelium lens fiber cell whole organism
Stage Range:	Prim-15 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	plod3^{tv205a/tv205a} plod3^vu222/vu222 plod3^tv205a/+; plod3^vu222/+
Observed In:	inner ear lens lens epithelium lens morphogenesis in camera-type eye whole organism
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 458(2), Taler, K., Weiss, O., Rotem, S., Rubinstein, A.M., Seritrakul, P., Gross, J.M., Inbal, A., Lysyl hydroxylase 3 is required for normal lens capsule formation and maintenance of lens epithelium integrity and fate, 177-188, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.