FIGURE

Fig. 5

ID

ZDB-FIG-200402-10

Publication

Ma et al., 2019 - A Comprehensive Study of High Cholesterol Diet-Induced Larval Zebrafish Model: A Short-Time In Vivo Screening Method for Non-Alcoholic Fatty Liver Disease Drugs

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Fig. 5

Further evaluation of HCD induced 2 weeks larval zebrafish model by bezafibrate and pioglitazone treatments. (A) Nile red stain and ROS production of Larval zebrafish. (B) Oxidant stress index of ROS, MDA, and SOD of larval zebrafish (n=6). (C) Oil red O staining of larval zebrafish, the hepatic steatosis was point out with yellow circle. (D) HE staining of larval zebrafish liver, macrovesicular steatosis, and microvesicular steatosis were point out with red arrows. (E) Macrovesicular steatosis area percentage of larval zebrafish liver (evaluated by 6 experts). (F)mRNA expression of lipogenesis and lipid-lowering of larval zebrafish (n=6). (G) mRNA expression of inflammation, Fibrosis and oxidant stress of larval zebrafish (n=6). Bar indicate means ± SD. n.s.P >0.05, ***P<0.001 represent significance of difference, P<0.05 was considered as statistically significant. Significant was calculated by ANOVA followed by turkey's test. n indicates the replicates of experiment.

Expression Data

Genes:	cpt1aa fasn il1b keap1a mmp9 nfe2l2a pparab pparg srebf1 tgfb2 tnfa
Fish:	WT
Conditions:	chemical treatment: bezafibrate, chemical treatment by diet: cholesterol chemical treatment by diet: cholesterol chemical treatment: pioglitazone, chemical treatment by diet: cholesterol
Anatomical Term:	whole organism
Stage:	Days 14-20

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Conditions:	chemical treatment: bezafibrate, chemical treatment by diet: cholesterol chemical treatment by diet: cholesterol chemical treatment: pioglitazone, chemical treatment by diet: cholesterol
Observed In:	liver fatty acid liver vesicle superoxide dismutase activity whole organism whole organism malonaldehyde whole organism reactive oxygen species whole organism triglyceride
Stage:	Days 14-20

Phenotype Detail

Acknowledgments

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