FIGURE

Fig. 2

ID

ZDB-FIG-200326-33

Publication

Giusti et al., 2019 - Inhibition of the thyroid hormonogenic H₂O₂ production by Duox/DuoxA in zebrafish reveals VAS2870 as a new goitrogenic compound

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Fig. 2

Overstimulation of the pituitary-thyroid axis in 120 hpf hypothyroid duox sa13017 mutant larvae (A) Schematic representation of the pituitary-thyroid axis with the thyroid hormones (TH)-mediated negative feedback loop inhibiting thyrotropin (TSH) secretion. (B) Whole-mount in situ hybridization (WISH) showed that larvae presenting a goitrous phenotype have increased tshb mRNA expression in the pituitary gland (arrow heads) compared to unaffected siblings. Images at the top show dorsal views of whole embryos (anterior to the left, scale bars: 100 μm). Images at the bottom show higher magnification views of the pituitary in coronal sections (anterior to the left, scale bars: 50 μm). (C) At the thyroid level (arrow heads), increased expression of TSH-dependent functional genes (tg, slc5a5/nis, tpo, duox and duoxa) was detected by WISH in larvae with a goitrous thyroid. Head region of representative larvae are shown in ventral views (anterior to the left, scale bars: 100 μm). (D) Fold change (mean ± SD, n = 3) of tg, slc5a5/nis, tpo and tshb mRNA expression in goiter affected animals compared to normal (WT or heterozygous mutants) specimens.

Expression Data

Genes:	duox duox2 slc5a5 tg tpo tshba
Fish:	duox^{sa13017/sa13017}; mitfa^w2/w2; mpv17^a9/a9; ulb4Tg
Anatomical Terms:	hypophysis thyroid follicle
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	duox^{sa13017/sa13017}; mitfa^w2/w2; mpv17^a9/a9; ulb4Tg
Observed In:	hypophysis thyroid follicle
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Molecular and Cellular Endocrinology, 500, Giusti, N., Gillotay, P., Trubiroha, A., Opitz, R., Dumont, J.E., Costagliola, S., De Deken, X., Inhibition of the thyroid hormonogenic H₂O₂ production by Duox/DuoxA in zebrafish reveals VAS2870 as a new goitrogenic compound, 110635, Copyright (2019) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.