FIGURE

Fig. S1

ID

ZDB-FIG-200326-30

Publication

Giusti et al., 2019 - Inhibition of the thyroid hormonogenic H₂O₂ production by Duox/DuoxA in zebrafish reveals VAS2870 as a new goitrogenic compound

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Fig. S1

L-T4 supplementation of duox sa13017 mutant larvae. The progeny of heterozygous duox sa13017 fish (Tg(tg:nlsEGFP) background) were selected based on thyroid phenotypes at 100 hpf and corresponding larvae were treated or not with 11 nM L-thyroxine (L-T4) until 144 hpf to be analyzed. (A) Epifluorescence live imaging of thyroidal EGFP signal. Representative images (ventral view of the head region, anterior to the left, scale bars: 75 μm) showing a marked decrease of the EFGP signal in L-T4 treated animals. (B) Comparative fold change mRNA expression of tg, slc5a5/nis, tpo and tshb genes compared to normal (WT or heterozygous mutants) non-treated animals. The means with SD are presented on the graphs (n = 3). Mann-Whitney tests were performed to compare normal non-treated larvae with all other conditions.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	duox^{sa13017/sa13017}; mitfa^w2/w2; mpv17^a9/a9; ulb4Tg
Observed In:	thyroid follicle
Stage:	Day 6

Phenotype Detail

Acknowledgments

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Reprinted from Molecular and Cellular Endocrinology, 500, Giusti, N., Gillotay, P., Trubiroha, A., Opitz, R., Dumont, J.E., Costagliola, S., De Deken, X., Inhibition of the thyroid hormonogenic H₂O₂ production by Duox/DuoxA in zebrafish reveals VAS2870 as a new goitrogenic compound, 110635, Copyright (2019) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.