FIGURE

Fig. S3

ID

ZDB-FIG-200326-31

Publication

Giusti et al., 2019 - Inhibition of the thyroid hormonogenic H₂O₂ production by Duox/DuoxA in zebrafish reveals VAS2870 as a new goitrogenic compound

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Fig. S3

Overstimulation of the pituitary-thyroid axis in 120 hpf hypothyroid duoxa mutant larvae. Heterozygous duoxa Δ11nt mutant fish (in Tg(tg:nlsEGFP) background) were mated and the progeny raised to 120 hpf for live imaging. Larvae were sorted according to thyroid phenotype (normal or goiter). (A) Fold change (mean ± SD, n = 3) of tg, slc5a5/nis, tpo and tshb mRNA expression in goiter affected animals compared to normal (WT or heterozygous mutants) specimens. (B) Increase of tshb mRNA expression by WISH in the pituitary gland of larvae presenting a goitrous phenotype compared to unaffected siblings. Representative coronal sections are shown (dorsal view, anterior to the left, scale bars: 50 μm). (C) At the thyroid level, increased expression of TSH-dependent functional genes (tg, slc5a5/nis, tpo, duox and duoxa) was detected (arrow heads) by WISH in larvae with a goitrous thyroid. Representative larvae are shown in ventral views of the head region (anterior to the left). Scale bars: 100 μm.

Expression Data

Genes:	duox duox2 slc5a5 tg tpo tshba
Fish:	duox2^ulb5/ulb5; ulb4Tg
Anatomical Terms:	hypophysis thyroid follicle
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	duox2^ulb5/ulb5; ulb4Tg
Observed In:	hypophysis thyroid follicle
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Molecular and Cellular Endocrinology, 500, Giusti, N., Gillotay, P., Trubiroha, A., Opitz, R., Dumont, J.E., Costagliola, S., De Deken, X., Inhibition of the thyroid hormonogenic H₂O₂ production by Duox/DuoxA in zebrafish reveals VAS2870 as a new goitrogenic compound, 110635, Copyright (2019) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.