FIGURE

Figure 5

ID

ZDB-FIG-200130-19

Publication

Chen et al., 2019 - Lysophosphatidic acid receptor LPA₃ prevents oxidative stress and cellular senescence in Hutchinson-Gilford progeria syndrome

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Figure 5

LPA₃ deficiency in zebrafish leads to premature aging phenotypes. (a) Kaplan–Meier survival analysis demonstrated a shorter lifespan in Lpa₃^−/− zebrafish than in corresponding WT siblings (p value = .011, N > 8). (b) Histological analysis shows hematopoietic stem cells loss in kidney marrow of 6‐month‐old Lpa₃^−/− zebrafish. (c) Sperm loss in the testis. (d) Bile duct hyperplasia. (e) Islet hyperplasia in the pancreas. (f) By senescence‐associated β‐gal staining assay, Lpa₃^−/− larvae show a positive β‐gal signal. (g) Representative images of zebrafish swimming tracks obtained and analyzed by locomotor. Locomotor results show that Lpa₃^−/− larvae had lower mobility than WT upon drastic light onset and offset. (h) CM‐DCFDA staining showed increased ROS levels in 5 dpf Lpa₃^−/− larvae. (i) Real‐time qPCR showed enhanced p16 and p21, whereas reduced Gpx1a, SOD2, and NQO1 of 5 dpf Lpa₃^−/− larvae. β‐Actin was used as internal control. ANOVA and Student's t test; *p < .05, ***p < .001

Expression Data

Genes:	cdkn1a cdkn2a/b gpx1a il6 nqo1 sod2
Fish:	WT lpar3^cy52/cy52
Anatomical Term:	whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lpar3^cy52/cy52
Observed In:	bile ductule head kidney hematopoietic stem cell islet sperm whole organism whole organism reactive oxygen species
Stage Range:	Day 5 to Adult

Phenotype Detail

Acknowledgments

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