FIGURE

Figure 4

ID

ZDB-FIG-200125-58

Publication

Matchett et al., 2019 - Paralogues of Mmp11 and Timp4 Interact during the Development of the Myotendinous Junction in the Zebrafish Embryo

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Figure 4

Timp4 and Mmp11 paralogues accumulate dynamically during development and co-localize at the myotendinous junctions (MTJs). Composite confocal projections of 24, 36, 48, 72 and 96 hpf embryos labeled with antibodies against Timp4, Mmp11a, or Mmp11b. (A–E) Timp4 immunoreactivity is abundant at the MTJ at all stages examined (arrowhead), and accumulates dynamically in ectodermal epithelia of the head, fin folds, otic vesicle, the posterior notochord and muscle attachments in the jaws and pectoral fins in later stages. (F–J) Mmp11a is present at the MTJs in 24 hpf embryos (F) (arrowhead) but becomes localized within muscle cells in later stages (G,H). (K–O) Mmp11b becomes concentrated in MTJs at later stages (L–O) (arrowhead). Insets show higher magnification views of MTJs dorsal to the yolk extension in 24 and 96 hpf embryos for comparison. Scale bar = 500 µm.

Expression Data

Genes:	mmp11a mmp11b
Antibodies:	Ab1-mmp11a Ab1-mmp11b Ab1-timp4
Fish:	TU
Anatomical Terms:	fin ecto-epithelial cell hatching gland hatching gland cell head ecto-epithelial cell lens median fin fold mesenchyme pectoral fin myoseptum muscle tendon junction myotome otic vesicle pectoral fin tendon pectoral fin fold pharyngeal arch 1 pharyngeal arch 1 tendon solid lens vesicle somite border
Stage Range:	Prim-5 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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