FIGURE

Figure 2

ID

ZDB-FIG-200125-56

Publication

Matchett et al., 2019 - Paralogues of Mmp11 and Timp4 Interact during the Development of the Myotendinous Junction in the Zebrafish Embryo

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Figure 2

Schematic illustration of the construction of EMMA–Mmp11a. The coding sequence of zebrafish mmp11a 3′ to the secretory signal was amplified using primers with Gateway recombination sites, recombined into a donor vector, and from there into the EMMA destination vector. This allows the expression of a doubly epitope-tagged protein, whereby loss of co-localization of the HA and GFP epitopes signifies proteolytic activation of the protease by endogenous mechanisms [25].

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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