Fig. 1-S2
- ID
- ZDB-FIG-200106-7
- Publication
- Jiang et al., 2019 - A conserved morphogenetic mechanism for epidermal ensheathment of nociceptive sensory neurites
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c4da neurons are enclosed by epidermal sheaths.( A–C) Double labeling of sensory neurons (HRP) and epidermal sheaths (PLCδ-PH-GFP fluorescence) stained under non-permeabilizing conditions. Maximum projection images show double labeling ( A), epidermal PLCδ-PH-GFP signal intensity ( B), and HRP surface staining intensity ( C). Dashed lines mark sheath segments for which intensity profiles are shown in ( D, E). Note the apparent reciprocal relationship between surface-exposed HRP and PLCδ-PH-GFP intensities. ( F, G) Scatterplots show the relationship between surface-exposed HRP and PLCδ-PH-GFP signal intensities on sheaths from eight individual neurons at 84 h after egg laying (AEL), shortly after sheath formation is initiated ( F) and at 120 h AEL, when c4da neurons are extensively ensheathed ( G). Note the inverse linear regression. |