Figure 5
- ID
- ZDB-FIG-190723-2685
- Publication
- Jiang et al., 2019 - A conserved morphogenetic mechanism for epidermal ensheathment of nociceptive sensory neurites
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- Fig. 1
- Fig. 1-S1
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- Figure 5
- Figure 5—figure supplement 1.
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- Figure 6
- Figure 6—figure supplement 1.
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( A–D) Time of arrival of PIP2 and other sheath markers. Images show dual labeling of sheaths by PLCδ-PH-Cerulean and dArf6-GFP ( A) or GMA-GFP to label F-actin ( C) in larvae additionally expressing the c4da-specific marker ppk-CD4-tdTomato. ( B, D) Plots show mean and standard deviation values for the proportion of c4da dendrite arbors ensheathed by structures labeled by the indicated markers at the indicated times. All sheath structures labeled by dArf6-GFP and GMA-GFP were labeled by PLCδ-PH-Cerulean. ( E) Time-lapse images show dual labeling of sheaths by PLCδ-PH-Cerulean and GFP-cora1-383 in larvae additionally expressing the c4da-specific marker ppk-CD4-tdTomato. Cyan arrows mark single-positive (Cerulean-positive) sheaths that convert to double-positive (Cerulean-positive and GFP-positive), magenta arrows mark double-positive sheaths that persist. See also Figure 5—figure supplement 1 for time-lapse images depicting dArf6-GFP and GMA-GFP recruitment to PLCδ-PH-Cerulean sheaths. ( F) Quantification of marker recruitment. Bars depict the proportion of PLCδ-PH-Cerulean-positive GFP-negative sheaths at 104 h AEL that are labeled by the indicated markers at 120 h AEL. More than 100 sheaths (from six independent time-lapse series) were scored for each marker combination. ( G) Timing of accumulation of ensheathment channel markers in the zebrafish epidermis. ( H) Epistatic relationship between markers. The indicated RNAi transgenes were expressed in the epidermis and effects on ensheathment were assessed (see Figure 5—figure supplement 2 for accompanying images). Plots show mean and standard deviation values for the proportion of c4da dendrite arbors wrapped by PLCδ-PH-GFP or cora-positive sheaths. n = 8 neurons each; ***p<0.001 relative to control; one way ANOVA with post-hoc Dunnett’s test. ( I) Model depicting the timing of arrival of sheath components. |