FIGURE

Fig. 4.

ID

ZDB-FIG-191230-496

Publication

Ding et al., 2019 - Haploinsufficiency of mechanistic target of rapamycin ameliorates bag3 cardiomyopathy in adult zebrafish

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Fig. 4.

Transcriptome analysis identifies mTOR as one of the top signaling pathways affected in the bag3^e2/e2 mutant. (A) Numbers of differentially expressed (DE) genes between bag3^e2/e2 mutant hearts and WT control with a cutoff of adjusted P<0.05. (B) Heat map presenting the expression of 5361 DE genes between the bag3^e2/e2 mutant and WT control. Gene expression levels are shown in log₁₀ [reads per kilobase per million reads (RPKM)]. (C) The top 10 DE gene enriched pathways with significant P-values suggested by IPA. The red column shows that the mTOR pathway was one of the top pathways altered and was subsequently experimentally tested. (D) Representative western blot images and quantification analysis of the expression levels of mTOR downstream proteins. LC3-II protein was examined in heart tissues from fish treated with 50 nM bafilomycin A1 for 4 h. n=4 biological replicates, Student's t-test. Data are mean±s.e.m.

Expression Data

Antibodies:	Ab1-eif4ebp1 Ab1-map1lc3 Ab1-rps6 Ab2-eif4ebp1 Ab5-rps6
Fish:	WT bag3^xu083/xu083
Anatomical Term:	heart
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	bag3^xu083/xu083
Observed In:	heart
Stage:	Adult

Phenotype Detail

Acknowledgments

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