FIGURE

Figure 3

ID

ZDB-FIG-191230-1092

Publication

Chen et al., 2019 - Pyridoxamine Supplementation Effectively Reverses the Abnormal Phenotypes of Zebrafish Larvae With PNPO Deficiency

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Figure 3

Spatial and temporal distribution of zebrafish pnpo. The expression profiles of pnpo in zebrafish at different developmental stages and tissues in adult fish were examined by RT-PCR and WISH. Embryos at the (A) indicated stages and (B) tissues of adult female zebrafish were extracted for total mRNA and subjected to RT-PCR with zpnpo-specific primers. The amount of zpnpo transcripts was normalized with zebrafish β-actin. Results presented are the averages shown in mean ± SEM from at least three independent repeats. (C) Embryos at various developmental stages were examined with WISH by probing with the DIG-labeled riboprobes specific to zpnpo as described in the Materials and Methods. RT-PCR, reverse transcription–polymerase chain reaction; WISH, whole-mount in situ hybridization; DIG, digoxigenin.

Expression Data

Gene:	pnpo
Fish:	AB
Anatomical Terms:	brain digestive system eye female organism heart liver muscle oocyte spinal cord spleen whole organism
Stage Range:	2-cell to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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