Figure 6

Rescue of zPnpo morphant morphology. Embryos injected with 5 ng of zPnpo MO at one- to two-cell stage were co-injected with 800 pg of zpnpo or hPNPO mRNA (columns 4 and 5) or grown in embryo water containing 1 mM of PLP, 1 mM of PN, 0.1 mM of PM, or 0.1 mM of PL (columns 6–9). Embryos injected with 10 ng of scrambled control MO were used as control (CTL). Development of various tissues was examined under light dissecting microscope at 3 dpf for brain (A), eyes (B), and heart (C); at 4 dpf for trunk (body curvature) (D); and at 5 dpf for swim bladder (E). The larvae, displaying the morphology comparable with that of wild-type control of the same stage for the characterized tissue/organ, were categorized as normal, and the percent of larvae displaying normal morphology in each group was recorded. (F) The survival rate of zPnpo morphants was recorded at 4 dpf. The averages of data are reported from at least three independent repeats with n = 60∼80 embryos for each group and expressed as mean ± SEM. The statistical significance was calculated with one-tailed Mann–Whitney nonparametric U test by comparing the experimental groups with wild type (*) or with zPnpo MO (#). “*^/#p < 0.05; **^/##p < 0.01. MO, morpholino oligonucleotide; PLP, pyridoxal 5′-phosphate; PN, pyridoxine; PM, pyridoxamine; PL, pyridoxal.