FIGURE

Fig. 6

ID

ZDB-FIG-191119-4

Publication

Große et al., 2019 - Zebrafish Wtx is a negative regulator of Wnt signaling but is dispensable for embryonic development and organ homeostasis

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Fig. 6

Wtx/amer2/amer3 triple mutant embryos display dorsalization similar to wtxmorphants. Whole mount in situ hybridization for expression analysis of markers for lateral mesoderm and neuroectoderm. A mixture of pax2a, myod1 and six3bprobes was applied to wild type and wtx/amer2/amer3 triple mutant embryos. The mutants display an expansion of the respective expression domains (white arrowheads) in the midbrain‐hindbrain boundary ( pax2a), optic primordia ( six3b) and in the somites ( myod1). Triple mutants injected with a wtx morpholino (ATG) have similarly expanded expression domains. Embryos were analyzed at 12 hpf and are shown with anterior to the left in a cranial as well as in a dorsal view. For “wild type uninjected”, “triple mutant uninjected” and “triple mutant wtx (ATG) MO” 17, 19, and 13 embryos were analyzed, respectively. EF, eye field; MHB, midbrain‐hindbrain boundary; PM, presomitic mesoderm; SM, somatic mesoderm; SO, somites. Scale bar represents 200 μm

Expression Data

Genes:	myod1 pax2a six3b
Fish:	WT amer1^li17/li17; amer2^li18/li18; amer3^li20/li20 amer1^li17/li17; amer2^li18/li18; amer3^li20/li20 + MO3-amer1
Knockdown Reagent:	MO3-amer1
Anatomical Terms:	anterior neural rod midbrain hindbrain boundary neural plate segmental plate somite
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	amer1^li17/li17; amer2^li18/li18; amer3^li20/li20 amer1^li17/li17; amer2^li18/li18; amer3^li20/li20 + MO3-amer1
Knockdown Reagent:	MO3-amer1
Observed In:	anterior neural rod midbrain hindbrain boundary neural plate somite
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

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