ZFIN Image: Große et al., 2019, Fig. 6

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Figure Caption

Fig. 6

Wtx/amer2/amer3 triple mutant embryos display dorsalization similar to wtxmorphants. Whole mount in situ hybridization for expression analysis of markers for lateral mesoderm and neuroectoderm. A mixture of pax2a, myod1 and six3bprobes was applied to wild type and wtx/amer2/amer3 triple mutant embryos. The mutants display an expansion of the respective expression domains (white arrowheads) in the midbrain‐hindbrain boundary ( pax2a), optic primordia ( six3b) and in the somites ( myod1). Triple mutants injected with a wtx morpholino (ATG) have similarly expanded expression domains. Embryos were analyzed at 12 hpf and are shown with anterior to the left in a cranial as well as in a dorsal view. For “wild type uninjected”, “triple mutant uninjected” and “triple mutant wtx (ATG) MO” 17, 19, and 13 embryos were analyzed, respectively. EF, eye field; MHB, midbrain‐hindbrain boundary; PM, presomitic mesoderm; SM, somatic mesoderm; SO, somites. Scale bar represents 200 μm

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Acknowledgments

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