FIGURE

Fig. 5

ID

ZDB-FIG-190701-12

Publication

Cal et al., 2019 - Countershading in zebrafish results from an Asip1 controlled dorsoventral gradient of pigment cell differentiation

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Fig. 5

Detailed visualization of ventral pigment cells in WT and asip1 mutants. (A) Ventral view of 210 dpf WT belly. (B) Belly of 210 dpf WT fish carrying Tg(Kita:GalTA4;UAS:mCherry) (labels melanophores) transgene shows no melanophores in ventral skin. (C) Ventral view of 210 dpf asip1^K.O. belly. (D) Belly of 210 dpf asip1^K.O. fish carrying Tg(Kita:GalTA4;UAS:mCherry) transgene shows high number of melanophores in ventral skin. (E) Internal view of 210 dpf WT abdominal wall shows a white sheet of iridophores with few internal melanophores (black arrow). (F) Abdominal wall of 210 dpf WT fish carrying Tg(TDL358:GFP) (labels iridophores and glia) transgene displays a uniform and continuous sheet of iridophores. (G) Internal view of 210 dpf asip1^K.O. abdominal wall shows a disrupted and discontinuous sheet of iridophores with high number of melanophores (black arrow) and some xanthophores (orange arrow). (H) Abdominal wall of 210 dpf asip1^K.O. fish carrying Tg(TDL358:GFP)transgene exhibits a broken sheet of iridophores. Scale bars: 100 μm.

Expression Data

Genes:	GFP mCherry
Fish:	hzm1Et tdl358Et asip1^iim08/iim08; hzm1Et asip1^iim08/iim08; tdl358Et
Anatomical Terms:	glial cell iridophore melanocyte
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	asip1^iim08/iim08; tdl358Et
Observed In:	iridophore melanocyte trunk ventral surface xanthophore
Stage:	Adult

Phenotype Detail

Acknowledgments

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