Fig. 4

Quantitation of dorsal-ventral distribution of melanophores and xanthophores in adult WT and asip1^K.O.fish. (A) Lateral view of zebrafish showing the body regions selected for melanophore and xanthophore count. (B) Ventral view of the WT and asip1^K.O. 210 dpf zebrafish fish belly. (C) Distribution and number of melanophores in WT and asip1^K.O. 60 dpf fish. At this stage, asip1^K.O. shows a significantly higher number of melanophores in the black stripe 2 V, ventral head and belly. (D) Number of xanthophores in the dorsal and ventral skin of WT and asip1^K.O. 60 dpf fish. At this stage, asip1^K.O. shows a significantly higher number of xanthophores in the belly region. (E) Distribution and number of melanophores in WT and asip1^K.O. 210 dpf fish. At this stage, asip1^K.O. shows significantly higher number of melanophores also in black stripe 2 V, 3 V, ventral head and belly. (F) Number of xanthophores in dorsal and ventral skin of WT and asip1^K.O. 210 dpf fish. These fish showed highly significant higher number of xanthophores in belly region than WT. Data are the mean ± SEM, n = 7. Asterisks indicate significant differences between WT and asip1^K.O. fish. Scale bar (A,C,E) 1 mm, (B) 100 μm.