FIGURE

Fig. 1

ID

ZDB-FIG-190618-27

Publication

Park et al., 2019 - 3-ketodihydrosphingosine reductase mutation induces steatosis and hepatic injury in zebrafish

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Fig. 1

Progression of liver injury in the Kdsr^I105R zebrafish mutant. Whole mount oil-red O (ORO) staining in wild type control sibling (A, left) and kdsr mutant (B, left) at 6 days post fertilization (dpf) and 7 dpf. ORO staining performed in transverse section of the liver (L, right panels of A and B). N = 10 per each. Lipid accumulation in the heart (h) and blood vessel (bv) are marked in images of panel B. H & E staining results in control at 7 dpf (left), mutant at 7 dpf (middle), and mutant at 8 dpf (right) (C, n = 6 per each). The magnified area depicts hepatocyte ballooning in mutants at 7 dpf. Images shown are representative of at least 10 other zebrafish or livers, respectively. Scale bars = 100 µm (whole mount ORO staining in A and B), 40 µm (ORO staining on the liver section in A and B), and 100 µm (H&E staining in C). The predicted protein structure of kdsr and the locus of the mutated amino acid is shown in (D). Relative mRNA expression of tnfa, il1b, and col1a1a in the wild type and mutant siblings is shown in (E). *P ≤ 0.05.

Expression Data

Genes:	col1a1a il1b tnfa
Fish:	AB/TU kdsr^{muz106/muz106} (AB/TU)
Anatomical Term:	whole organism
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	kdsr^{muz106/muz106} (AB/TU)
Observed In:	heart lipid hepatocyte liver lipid liver and biliary system blood vessel whole organism
Stage Range:	Day 6 to Days 7-13

Phenotype Detail

Acknowledgments

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